Even though short-term experiments toxicity assays, such as<br>the colorimetric assays, are usually not indicated towards monitoring of toxicity in degradation by-products, they allowed the<br>precise modelling and quantification of AFFF effects on the soil<br>microbiota. Phyto-toxicity assays further expanded the AFFF environmental safety assessment since they are operated on a much<br>longer period, observing the appearance and disappearance of certain metabolites with an impact on toxicity. We observed that both<br>techniques describe the AFFF environmental release scenario not<br>only with a long-term decrease in toxicity, but also with an actual<br>retardation of microbial metabolism in soil. Future studies are necessary to determine via further chemical analysis in plant tissues<br>which bioconcentrated compounds result from AFFF metabolism<br>in the environment. As plants were observed capable of growth in<br>AFFF contaminated environment after 240 days, bioaccumulation<br>of PFCs in the both test organisms (arugula and lettuce) is a likely<br>assumption.
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